*Combined femtosecond upconversion and TCSPC measurement in a small footprint
*Straightforward operation and easy day‑to‑day maintenance
*Works as an add‑on to a HARPIA‑TA or as a standalone unit
*Easy switching between fluorescence upconversion and TCSPC modes
*Compatible with PHAROS and CARBIDE series lasers running at 50 – 1000 kHz
*Analog PMT detector option for fluorescence upconversion
*Automated spectral scanning and calibration of upconversion crystal and prism
*Measurement of fluorescence dynamics in the femtosecond to microsecond range
*Full control over the following parameters of the pump beam:
– Polarization (using a Berek polarization compensator)
– Intensity (using manual or automated continuously variable neutral density filters)
- Gate delay (using an optical delay line)
*Spectrally‑resolved fluorescence detection using a monochromator
*When combined with a HARPIA‑TA main unit, a single monochromator can be used for both time‑resolved absorption and fluorescence measurements with no detector swapping necessary. Other monochromator options are available, such as a double subtractive monochromator for higher TCSPC time resolution
The HARPIA‑TF is a time‑resolved fluorescence measurement module which combines fluorescence upconversion and TCSPC techniques. In fluorescence upconversion, the signal from the sample is mixed in a nonlinear crystal with a gating femtosecond pulse to achieve high temporal resolution, which is limited by the duration of the gate pulse and is in the range of 250 fs. For fluorescence decay times exceeding 150 ps, the instrument can be used in time‑correlated single‑photon counting (TCSPC) mode to measure kinetic traces in the 200 ps – 2 μs range. The HARPIA‑TF module supports Becker&Hickl TCSPC devices and detectors.
The combination of these two time‑resolved fluorescence techniques enables the measurement of spectrally‑-resolved fluorescence decay in the femtosecond to microsecond range.
With the use of a high repetition rate PHAROS or CARBIDE laser, the fluorescence dynamics can be measured while exciting the samples with low pulse energies down to several nanojoules.
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